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DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]

DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate]?Features and Biological ApplicationsDiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluores...

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DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate]

Features and Biological Applications

DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He–Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

目錄號	產(chǎn)品名稱	規(guī)格	分子量	Ex/Em	推薦濾光器*?
DiD
22031	DiD?iodide	25mg	959.91	644/663 nm?	XF47-Omega, 31023-Chroma?
22033	DiD labeling solution	10 mL	959.91	644/663 nm?	XF47-Omega, 31023-Chroma?
DiO
22046	DiOC6(3) iodide	100 mg	572.52	482/504 nm?	XF23-Omega, 31001-Chroma?
22066	DiO perchlorate	100 mg	881.7	484/501nm	XF23-Omega, 31001-Chroma?
DiI
22101	DiI iodide	100 mg	961.32	549/565 nm?	XF32-Omega, 31002-Chroma?
22102	DiI perchlorate	100 mg	933.87	549/565 nm?	XF32-Omega, 31002-Chroma?
22103	DiI triflate	100 mg	983.48	549/565 nm?	XF32-Omega, 31002-Chroma?
DiR**
22070	DiR iodide	10 mg	1013.39	748/780 nm?	XF112-Omega, 41009-Chroma?
DiS
22073	DiSC2(3)	25 mg	492.44	560/571 nm?	XF32-Omega, 31002-Chroma?
22076	DiSC3(5)	100 mg	546.53	660/675 nm?	XF47-Omega, 31023-Chroma?