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DiR碘化物

DiR碘化物

DiR小動物活體成像熒光試劑?產(chǎn)品名稱:?DiR(DiIC18(7)1,1’-dioctadecyltetramethylindotricarbocyanine?Iodide)分子式/分子量:C63H101IN2=1013.4最大吸收波長/發(fā)射波長:?748/780?nm推薦濾光片設(shè)置:?710?ex/760?em結(jié)構(gòu)式：Figure?1.?T-cells isolated?from the spleen were fluorescently stained with DiR and i.v. injected (5x106?cells/mouse)?into a Nu/Nu mouse. Images above taken 24hrs post injection with IVIS Spectrum??show cells homing to the spleen.?DiR染料是親脂性的近紅外花菁熒光染料，可以用來染細(xì)...

DiR小動物活體成像熒光試劑


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產(chǎn)品名稱:?DiR(DiIC18(7)1,1’-dioctadecyltetramethylindotricarbocyanine?Iodide)

分子式/分子量:C63H101IN2=1013.4

最大吸收波長/發(fā)射波長:?748/780?nm

推薦濾光片設(shè)置:?710?ex/760?em

結(jié)構(gòu)式：

Figure?1.?T-cells isolated?from the spleen were fluorescently stained with DiR and i.v. injected (5x106?cells/mouse)?into a Nu/Nu mouse. Images above taken 24hrs post injection with IVIS Spectrum??show cells homing to the spleen.


?DiR染料是親脂性的近紅外花菁熒光染料，可以用來染細(xì)胞膜和其它脂溶性生物結(jié)構(gòu)。18個碳的長鏈插入細(xì)胞膜中從而對細(xì)胞進(jìn)行染色,?而細(xì)胞間的染料轉(zhuǎn)移可以忽略不計(jì)。DiR的發(fā)射的是近紅外熒光可以穿透細(xì)胞和組織，在活體成像中用來示蹤。

DIR一般對原代細(xì)胞進(jìn)行熒光染色并可進(jìn)行活體成像分布觀察, (例如下列細(xì)胞embryonic?stem cells,?bone marrow?derived?stem cells,?adipose?derived?stem cells,?lymphocytes and?erythrocytes),

? ? ? ?.?DiR?stock?was?prepared?by?dissolving?25?mg?in 3?mL?ethanol.?Working?solution?of?320?μg/mL?was?prepared?by?diluting?199?μL?of?stock?solution?in 5?mL?PBS.?T-cells?isolated?from?the?spleen?were?incubated?with?320?μg/mL?DiR.?After?30?min?incubation,?cells?were?spun?down?for 3?min?at?1000?rpm?at 4?oC?resulting?in a?blue?pellet.?Cells?were?washed?twice?in?PBS?and?injected?intravenously?(5 x?106?cells/mouse).?Control?group?was?injected?with 5 x?106?cells/mouse?in?PBS.?Mice?were?imaged?with?IVIS?Spectrum??at?10?min,?1hr,?6hr?and?24?hrs?post?injection.?Ideal?filter?set for?DiR?imaging?is?710?nm?excitation?and?760?nm?emission.?Mice?were?imaged?dorsally?as?well?as?ventrally?at?all?time?points.?Brain,?bones,?spleen,?liver,?lungs?and?kidneys?were?harvested?for?ex?vivo?imaging?24?hrs?post?injection.

Non-invasive?in?vivo?imaging?showed?the?homing?process?of?injected T?cells?to?the?liver?and?spleen?in?real?time,?which?was?confirmed?by?ex?vivo imaging.

參考文獻(xiàn)：? Kalchenko?et?al.,?Use?of?lipophilicnear-infrared?dye?in?whole-body?optical?imaging?of?hematopoietic?cell?homing.?Journal?of?Biomedical?optics,?September/October?2006,?Vol?11(5).


  1. 注意事項(xiàng)：本公司所有產(chǎn)品僅限用于研發(fā)，操作時必須戴口罩/手套/實(shí)驗(yàn)服和其它生化實(shí)驗(yàn)室防護(hù)措施。


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