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DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]

DiO perchlorate [3,3’-Dioctadecyloxacarbocyanine perchlorate]

DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate]?Features and Biological ApplicationsDiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluores...

DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate]

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Features and Biological Applications
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He–Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.


目錄號

產(chǎn)品名稱

規(guī)格

分子量

Ex/Em

推薦濾光器*?

DiD

22031

DiD?iodide

25mg

959.91

644/663 nm?

XF47-Omega, 31023-Chroma?

22033

DiD labeling solution

10 mL

959.91

644/663 nm?

XF47-Omega, 31023-Chroma?

DiO






22046

DiOC6(3) iodide

100 mg

572.52

482/504 nm?

XF23-Omega, 31001-Chroma?

22066

DiO perchlorate

100 mg

881.7

484/501nm

XF23-Omega, 31001-Chroma?

DiI

22101

DiI iodide

100 mg

961.32

549/565 nm?

XF32-Omega, 31002-Chroma?

22102

DiI perchlorate

100 mg

933.87

549/565 nm?

XF32-Omega, 31002-Chroma?

22103

DiI triflate

100 mg

983.48

549/565 nm?

XF32-Omega, 31002-Chroma?

DiR**

22070

DiR iodide

10 mg

1013.39

748/780 nm?

XF112-Omega, 41009-Chroma?

DiS

22073

DiSC2(3)

25 mg

492.44

560/571 nm?

XF32-Omega, 31002-Chroma?

22076

DiSC3(5)

100 mg

546.53

660/675 nm?

XF47-Omega, 31023-Chroma?

**DiR的發(fā)射波長肉眼不可見,所以需要配備CCD鏡頭或其他的近紅外檢測儀器。


References

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1.???Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ. (2006) Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials. J Biomed Mater Res B Appl Biomater.

2.???Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K. (2006) In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope. Invest Ophthalmol Vis Sci, 47, 2943.

3.???Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A. (2006) Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. J Biomed Opt, 11, 050507.

4.???Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C. (2006) Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius. Hypertension, 48, 482.

5.???Wertz A, Rossler W, Obermayer M, Bickmeyer U. (2006) Functional neuroanatomy of the rhinophore of Aplysia punctata. Front Zool, 3, 6.

6.???Harrison TA, Perry KM, Hoover DB. (2005) Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing. Anat Rec A Discov Mol Cell Evol Biol, 285, 758.

7.???Huesa G, Anadon R, Yanez J. (2003) Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study. J Comp Neurol, 460, 327.

8.???Kiyohara Y, Endo K, Ide C, Mizoguchi A. (2003) A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle. J Electron Microsc (Tokyo), 52, 327.

9.???Levai O, Strotmann J. (2003) Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice. Histochem Cell Biol, 120, 483.


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