SulfoCy3
SulfoCy和LipoCy為北京富百科生物技術(shù)有限公司注冊商標，未經(jīng)授權(quán)嚴禁使用！
Structure
MW = 668.86? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?Spectra
?
Excitation and emission spectra of unconjugated SulfoCy3 dye in phosphate buffered saline.
?
Properties |
|
Excitation maximum (nm) |
548 |
Emission maximum (nm) |
562 |
Extinction coefficient (M-1 cm-1) |
150 000 * |
Quantum yield |
0.04 * |
Fluorescence lifetime (ns) |
<0.3 |
* Measured in phosphate-buffered saline, Mujumdar, R. B., Ernst, L. A., Mujumdar, S. R., Lewis, C. J., and Waggoner, A. S. (1993) Bioconjugate Chem. 4, 105-111.
(Note: The Exitation/Emission wavelength and quantum yield of Cy3 are changed when in a rigid microenvironment, for example in different pH, highly viscous solvents, or when conjugated to biomolecules).?Upon attachment to biomolecules (for example oligonucleotides), significant?shifts in excitation and emission wavelength have been observed ?under different?pH conditions. Cy3在不同的pH環(huán)境中，波長會有較大變化。
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