DiR小動(dòng)物活體成像熒光試劑

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產(chǎn)品名稱: DiR (DiIC18(7)1,1’-dioctadecyltetramethyl indotricarbocyanine Iodide)

分子式/分子量: C63H101IN2=1013.4

最大吸收波長/發(fā)射波長: 748/780 nm

推薦濾光片設(shè)置: 710 ex/760 em

結(jié)構(gòu)式：

Figure 1. T-cells isolated from the spleen were fluorescently stained with DiR and i.v. injected (5x106 cells/mouse) into a Nu/Nu mouse. Images above taken 24hrs post injection with IVIS Spectrum ?show cells homing to the spleen.

?DiR染料是親脂性的近紅外花菁熒光染料，可以用來染細(xì)胞膜和其它脂溶性生物結(jié)構(gòu)。18個(gè)碳的長鏈插入細(xì)胞膜中從而對(duì)細(xì)胞進(jìn)行染色, 而細(xì)胞間的染料轉(zhuǎn)移可以忽略不計(jì)。DiR的發(fā)射的是近紅外熒光可以穿透細(xì)胞和組織，在活體成像中用來示蹤。

DIR一般對(duì)原代細(xì)胞進(jìn)行熒光染色并可進(jìn)行活體成像分布觀察, (例如下列細(xì)胞embryonic stem cells, bone marrow derived stem cells, adipose derived stem cells, lymphocytes and erythrocytes),

?DiR stock was prepared by dissolving 25 mg in 3 mL ethanol. Working solution of 320 μg/mL was prepared by diluting 199 μL of stock solution in 5 mL PBS. T-cells isolated from the spleen were incubated with 320 μg/mL DiR. After 30 min incubation, cells were spun down for 3 min at 1000 rpm at 4 oC resulting in a blue pellet. Cells were washed twice in PBS and injected intravenously (5 x 106 cells/mouse). Control group was injected with 5 x 106 cells/mouse in PBS. Mice were imaged with IVIS Spectrum ?at 10 min, 1hr, 6hr and 24 hrs post injection. Ideal filter set for DiR imaging is 710 nm excitation and 760 nm emission. Mice were imaged dorsally as well as ventrally at all time points. Brain, bones, spleen, liver, lungs and kidneys were harvested for ex vivo imaging 24 hrs post injection.

Non-invasive in vivo imaging showed the homing process of injected T cells to the liver and spleen in real time, which was confirmed by ex vivo imaging.

參考文獻(xiàn)：? Kalchenko et al., Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. Journal of Biomedical optics, September/October 2006, Vol 11(5).

注意事項(xiàng)：本公司所有產(chǎn)品僅限用于研發(fā)，操作時(shí)必須戴口罩/手套/實(shí)驗(yàn)服和其它生化實(shí)驗(yàn)室防護(hù)措施。