?DPPH （2,2-Diphenyl-1-picrylhydrazyl）

??DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical that can be used to measure the radical scavenging activity of antioxidants. The odd electron of nitrogen atom in DPPH is reduced by receiving a hydrogen atom from antioxidants to the corresponding hydrazine. DPPH method may be utilized in aqueous and nonpolar organic solvents and can be used to examine both hydrophilic and lipophilic antioxidants.

? ?DPPH shows a strong absorption band at 517 nm due to its odd electron and solution appears a deep violet colour, the absorption vanishes as the electron pairs off. The resulting decolorization is stoichiometric with respect to the number of electrons taken up. The alcoholic solutions of 0.5 mM are densely colored and at this concentration, the Lambert-Beer law is obeyed over the useful range of absorption^[1].
DPPH assay is a rapid, simple, inexpensive and widely used method to measure the ability of compounds to act as free radical scavengers or hydrogen donors, and to evaluate antioxidant activity of foods. It can also be used to quantify antioxidants in complex biological systems, for solid or liquid samples. This method is easy and applies to measure the overall antioxidant capacity and the free radical scavenging activity of fruit and vegetable juice. This assay has been successfully utilized for investigating antioxidant properties of wheat grain and bran, vegetables, conjugated linoleic acids, herbs, edible seed oils, and flours in several different solvent systems including ethanol, aqueous acetone, methanol, aqueous alcohol and benzene. It is a convenient method for the antioxidant assay of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds, for olive oil, fruits, juices and wines.

Reference:

Kedare SB, et al. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011_48(4)_412-22.

NOTE:?Always wear lab coats, gloves and goggles when working with our products although?they are low-risk chemicals for R&D only.

DPPH (2,2-Diphenyl-1-picrylhydrazyl) 是一種穩(wěn)定的自由基，可用于測(cè)量抗氧化劑的自由基清除活性。DPPH 中氮原子的奇數(shù)電子通過(guò)從抗氧化劑吸收氫原子而還原為相應(yīng)的肼。DPPH 方法可用于水性和非極性有機(jī)溶劑中，并可用于檢查親水性和親脂性抗氧化劑。

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