ADHP / Amplex Red Reagent
Although chromogenic peroxidase substrates are widely used in a variety of biological detections, the low sensitity and narrow dynamic range limit their applications in the assays that require rapid, robust and sensitive detection of biological molecules.??Fluorimetric detection is known to be 2-3 magnitudes more sensitive than colorimetric detection. However, there are very few fluorogenic peroxidase substrates available.?
We are pleased to introduce our serieal fluorogenic substrates to address this unmet need. Our ADHP is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2?detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol,?L-glutamate, amino acids, etc.
Although ADHP exhibits very high sensitivity for detecting peroxidate activity either in a fluorimetric or colorimetric mode, it requires higher pH to reach its maximum fluorescence. We are pleased to introduce our ADHP that has better water solubility and stronger fluorescence than other ADHP. Addtionally, our ADHP?is the only fluorogenic peroxidase substrate that generates fluorescent precipitates upon the peroxidase-catalyzed oxidation.
? ?辣根過氧化酶HRP，由于其具有很好的穩(wěn)定性和低的分子量是用于組織化學(xué)和酶聯(lián)免疫檢測中極其重要的酶。雖然有不少顯色劑和發(fā)光劑的底物用于測定HRP，但是用于過氧化酶測定的熒光底物卻很少。
ADHP (10-acetyl-3,7-dihydroxyphenoxazine)?也稱為Amplex Red Reagent，是目前最穩(wěn)定和最敏感的HRP熒光底物。本試劑已將高純度的ADHP溶解于適當(dāng)?shù)木彌_溶液中，可直接用于HRP酶反應(yīng)，具有高靈敏度并且應(yīng)用方便。實踐證明，在ELISA實驗中，使用ADHP作為顯色底物，可檢測到最低濃度達(dá)10-15 M?的標(biāo)記物。
使用熒光酶標(biāo)儀檢測時請選擇：激發(fā)波長Ex=530-560 nm；發(fā)射波長Em =585 nm
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