6-HEX, SE [6-Carboxy-2',4,4',5',7,7'-hexachlorofluorescein, succinimidyl ester]?
HEX??is the registered trademark and strictly forbidden to be used without authorization.?
Cat# |
Size |
Price |
MW |
Abs |
Em |
Soluble in |
Storage? |
202 |
5 mg |
$145 |
680.06 |
533 nm |
550 nm |
DMSO |
F/D/L |
?
? |
Features and Biological Applications6-HEX, SE is the amine-reactive succinimidyl ester of 6-HEX acid. It is widely used in nucleic acid sequencing and related research. |
?References
1.??Andrieu-Soler C, Doat M, Halhal M, Keller N, Jonet L, BenEzra D, Behar-Cohen F. (2006) Enhanced oligonucleotide delivery to mouse retinal cells using iontophoresis. Mol Vis, 12, 1098.
2.??Pesquet E, Barbier O, Ranocha P, Jauneau A, Goffner D. (2004) Multiple gene detection by in situ RT-PCR in isolated plant cells and tissues. Plant J, 39, 947.
3.??Templeton KE, Scheltinga SA, Sillekens P, Crielaard JW, van Dam AP, Goossens H, Claas EC. (2003) Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species. J Clin Microbiol, 41, 4016.
4.??Iwamoto T, Sonobe T, Hayashi K. (2002) Novel algorithm identifies species in a polymycobacterial sample by fluorescence capillary electrophoresis-based single-strand conformation polymorphism analysis. J Clin Microbiol, 40, 4705.
5.??Fountain KJ, Gilar M, Budman Y, Gebler JC. (2003) Purification of dye-labeled oligonucleotides by ion-pair reversed-phase high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci, 783, 61.
6.Simeonov A, Nikiforov TT. (2002) Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection. Nucleic Acids Res, 30, e91.
7.??Reid SL, Parry D, Liu HH, Connolly BA. (2001) Binding and recognition of GATATC target sequences by the EcoRV restriction endonuclease: a study using fluorescent oligonucleotides and fluorescence polarization. Biochemistry, 40, 2484.
8.??Porter DJ, Preugschat F. (2000) Strand-separating activity of hepatitis C virus helicase in the absence of ATP. Biochemistry, 39, 5166.
9.Ryncarz AJ, Goddard J, Wald A, Huang ML, Roizman B, Corey L. (1999) Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples. J Clin Microbiol, 37, 1941.
10.???Powell LM, Connolly BA, Dryden DT. (1998) The DNA binding characteristics of the trimeric EcoKI methyltransferase and its partially assembled dimeric form determined by fluorescence polarisation and DNA footprinting. J Mol Biol, 283, 947.
11.???Gumbs OH, Shaner SL. (1998) Three mechanistic steps detected by FRET after presynaptic filament formation in homologous recombination. ATP hydrolysis required for release of oligonucleotide heteroduplex product from RecA. Biochemistry, 37, 11692.
12.???Porter DJ, Short SA, Hanlon MH, Preugschat F, Wilson JE, Willard DH, Jr., Consler TG. (1998) Product release is the major contributor to kcat for the hepatitis C virus helicase-catalyzed strand separation of short duplex DNA. J Biol Chem, 273, 18906.
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