5（6）-FAM
CAS#：72088-94-9
中文名：5(6)-羧基熒光素
英文名：5(6)-FAM [5-(and-6)-Carboxyfluorescein] *Mixed isomers*
分子式：C21H12O7
分子量：376.32
性質(zhì)：
1. 外觀：黃色粉末
2. 純度：≥95%（HPLC）
3. 產(chǎn)品描述：
用于標記蛋白、多肽、抗體及Oligo
儲存條件：-20℃干燥避光保存，有效期兩年。
5(6)-FAM [5-(and-6)-Carboxyfluorescein]
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Features and Biological ApplicationsCarboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. FITC-labeled nucleotides and peptides tend to deteriorate more quickly than the corresponding FAM conjugates. We found that FAM reagents can be used to substitute FITC reagents in most biological applications. |
References
1.?????Hahn M,?et al. (2001).??Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction-amplified short tandem repeats during denaturing capillary electrophoresis.?Electrophoresis22, 2691-700.
2.?????Hung SC,?et al. (1996).??Cyanine dyes with high absorption cross section as donor chromophores in energy transfer primers.?Anal Biochem243, 15-27.
3.????Banks PR and Paquette DM (1995). Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis.?Bioconjug Chem6, 447-458.
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