5-FAM
CAS#：76823-03-5
中文名：5-羧基熒光素
英文名：5-FAM [5-Carboxyfluorescein] *Single isomer*
分子式：C21H12O7
分子量：376.32
性質(zhì)：
1. 外觀：黃色粉末
2. 純度：≥95%（HPLC）
3. 產(chǎn)品描述：
用于標(biāo)記蛋白、多肽、抗體及Oligo
儲存條件：-20℃干燥避光保存，有效期兩年。
5-FAM [5-Carboxyfluorescein]
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Features and Biological Applications5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for??labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.? |
References
1.???Adamczyk, M., et al., Preparation of succinimidyl and pentafluorophenyl active esters of 5- and 6-carboxyfluorescein.Bioconjug Chem1997,?8, 253-5.
2.???Yefimov, S., et al., Sequential electroelution and mass spectroscopic identification of intact sodium dodecyl sulfate-proteins labeled with 5(6)-carboxyfluorescein-n-hydroxysuccinimide ester.Electrophoresis2001,?22, 2881-7.
3.???Walker, B., et al., Carboxyfluorescein and biotin neuromedin c analogues: Synthesis and applications.Peptides1995,?16, 255-61.
4.???Kemenes, G., et al., Photoinactivation of neurones axonally filled with the fluorescent dye 5(6)-carboxyfluorescein in the pond snail, lymnaea stagnalis.J Neurosci Methods1991,?39, 207-16.?
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